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normal cervical epithelial cell line ende1617  (ATCC)


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    ATCC normal cervical epithelial cell line ende1617
    IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in <t>ende1617,</t> HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times
    Normal Cervical Epithelial Cell Line Ende1617, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+cervical+epithelial+cell+line+ende1617/pmc10908604-51-7-23?v=ATCC
    Average 96 stars, based on 493 article reviews
    normal cervical epithelial cell line ende1617 - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Oct4 activates IL-17A to orchestrate M2 macrophage polarization and cervical cancer metastasis"

    Article Title: Oct4 activates IL-17A to orchestrate M2 macrophage polarization and cervical cancer metastasis

    Journal: Cancer Immunology, Immunotherapy

    doi: 10.1007/s00262-023-03596-z

    IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in ende1617, HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times
    Figure Legend Snippet: IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in ende1617, HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times

    Techniques Used: Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay, Expressing, Co-Culture Assay, Flow Cytometry



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    normal cervical epithelial cell line ende1617  (ATCC)
    96
    ATCC normal cervical epithelial cell line ende1617
    IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in <t>ende1617,</t> HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times
    Normal Cervical Epithelial Cell Line Ende1617, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+cervical+epithelial+cell+line+ende1617/pmc10908604-51-7-23?v=ATCC
    Average 96 stars, based on 1 article reviews
    normal cervical epithelial cell line ende1617 - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

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    IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in ende1617, HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times

    Journal: Cancer Immunology, Immunotherapy

    Article Title: Oct4 activates IL-17A to orchestrate M2 macrophage polarization and cervical cancer metastasis

    doi: 10.1007/s00262-023-03596-z

    Figure Lengend Snippet: IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in ende1617, HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times

    Article Snippet: Cervical cancer cell lines HeLa and SiHa, normal cervical epithelial cell line ende1617, and human monocytic cell line THP-1 all were purchased from ATCC (Manassas, VA).

    Techniques: Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay, Expressing, Co-Culture Assay, Flow Cytometry